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Illuminating the Function of the Hydroxyl Radical in the Brains of Mice with Depression Phenotypes by Two‐Photon Fluorescence Imaging
Author(s) -
Wang Xin,
Li Ping,
Ding Qi,
Wu Chuanchen,
Zhang Wen,
Tang Bo
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201901318
Subject(s) - oxidative stress , reactive oxygen species , hydroxyl radical , phenotype , chemistry , depression (economics) , fluorescence , fluorescence lifetime imaging microscopy , oxidative phosphorylation , biophysics , biochemistry , photochemistry , radical , gene , biology , physics , macroeconomics , quantum mechanics , economics
Depression is intimately linked with oxidative stress. As one of the most reactive and oxidative reactive oxygen species that is overproduced during oxidative stress, the hydroxyl radical ( . OH) can cause macromolecular damage and subsequent neurological diseases. However, due to the high reactivity and low concentration of . OH, precise exploration of . OH in brains remains a challenge. The two‐photon fluorescence probe MD‐B was developed for in situ . OH imaging in living systems. This probe achieves exceptional selectivity towards . OH through the one‐electron oxidation of 3‐methyl‐pyrazolone as a new specific recognition site. MD‐B can be used to map . OH in mouse brain, thereby revealing that increased . OH is positively correlated with the severity of depression phenotypes. Furthermore, . OH has been shown to inactivate deacetylase SIRT1, thereby leading to the occurrence and development of depression phenotypes. This work provides a new strategy for the future treatment of depression.