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Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O 2 to H 2 Production
Author(s) -
Su Dongyue,
Qi Jiarui,
Liu Xiaoman,
Wang Lei,
Zhang Hao,
Xie Hui,
Huang Xin
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201900255
Subject(s) - chlorella pyrenoidosa , chemistry , tannic acid , photosynthesis , enzyme , cell , biophysics , nanotechnology , chemical engineering , biochemistry , chlorella , materials science , botany , biology , organic chemistry , algae , engineering
Single‐cell encapsulation has become an effective strategy in cell surface engineering; however, the construction of cell wall‐like layers that allow the switching of the inherent functionality of the engineered cell is still rare. In this study, we show a universal way to create an enzyme‐modulated oxygen‐consuming sandwich‐like layer by using polydopamine, laccase, and tannic acid as building blocks, which then could generate an anaerobic microenvironment around the cell. This layer protected the encapsulated C. pyrenoidosa cell against external stresses and enabled it to switch from normal photosynthetic O 2 production to photobiological H 2 production. The layer showed an smaller effect on the PSII activity, which contributed a significant enhancement on the rate (0.32 μmol H 2 h −1 (mg chlorophyll) −1 ) and the duration (7 d) of H 2 production. This strategy is expected to provide a pathway for modulating the functionality of cells and for breakthroughs in the development of green energy alternatives.