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Simultaneous Visualization of Endogenous Homocysteine, Cysteine, Glutathione, and their Transformation through Different Fluorescence Channels
Author(s) -
Yin Guoxing,
Niu Tingting,
Yu Ting,
Gan Yabing,
Sun Xinyu,
Yin Peng,
Chen Haimin,
Zhang Youyu,
Li Haitao,
Yao Shouzhuo
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201813935
Subject(s) - cysteine , endogeny , glutathione , homocysteine , chemistry , fluorescence , transformation (genetics) , biophysics , visualization , biochemistry , biology , computer science , enzyme , physics , quantum mechanics , artificial intelligence , gene
Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases. Direct visualization of endogenous biothiols in biological systems is of great value to understanding their biological roles. Herein, a novel multi‐signal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine (Hcy), cysteine (Cys), and glutathione (GSH) using different emission channels. This probe was successfully applied to the simultaneous discrimination between and visualization of endogenous Hcy, Cys, GSH, and their transformation in living cells.