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A Minimal Sequence for Left‐Handed G‐Quadruplex Formation
Author(s) -
Bakalar Blaž,
Heddi Brahim,
Schmitt Emmanuelle,
Mechulam Yves,
Phan Anh Tuân
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201812628
Subject(s) - circular dichroism , g quadruplex , crystallography , dna , sequence (biology) , left handed , thymine , nuclear magnetic resonance spectroscopy , chemistry , dna sequencing , stereochemistry , structural motif , physics , biochemistry , optics
Recently, we observed the first example of a left‐handed G‐quadruplex structure formed by natural DNA, named Z‐G4 . We analysed the Z‐G4 structure and inspected its primary 28‐nt sequence in order to identify motifs that convey the unique left‐handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X‐ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left‐handed DNA G‐quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left‐handed G‐quadruplex formation.