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Photocatalysis Enables Visible‐Light Uncaging of Bioactive Molecules in Live Cells
Author(s) -
Wang Haoyan,
Li WeiGuang,
Zeng Kaixing,
Wu YanJiao,
Zhang Yixin,
Xu TianLe,
Chen Yiyun
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201811261
Subject(s) - chemistry , photochemistry , molecule , visible spectrum , photocatalysis , rhodamine b , eosin y , rhodamine , fluorescence , materials science , biochemistry , catalysis , organic chemistry , physics , optoelectronics , quantum mechanics
Abstract The photo‐manipulation of bioactive molecules provides unique advantages due to the high temporal and spatial precision of light. The first visible‐light uncaging reaction by photocatalytic deboronative hydroxylation in live cells is now demonstrated. Using Fluorescein and Rhodamine derivatives as photocatalysts and ascorbates as reductants, transient hydrogen peroxides were generated from molecular oxygen to uncage phenol, alcohol, and amine functional groups on bioactive molecules in bacteria and mammalian cells, including neurons. This effective visible‐light uncaging reaction enabled the light‐inducible protein expression, the photo‐manipulation of membrane potentials, and the subcellular‐specific photo‐release of small molecules.