Premium
A High‐Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis
Author(s) -
Zhang Le,
Liu Xin A.,
Gillis Kevin D.,
Glass Timothy E.
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201810919
Subject(s) - fluorophore , catecholamine , norepinephrine , exocytosis , total internal reflection fluorescence microscope , fluorescence , confocal microscopy , fluorescence microscope , confocal , chemistry , biophysics , biochemistry , microbiology and biotechnology , biology , membrane , endocrinology , optics , dopamine , physics
A fluorescent sensor for catecholamines, NS510, is presented. The sensor is based on a quinolone fluorophore incorporating a boronic acid recognition element that gives it high affinity for catecholamines and a turn‐on response to norepinephrine. The sensor results in punctate staining of norepinephrine‐enriched chromaffin cells visualized using confocal microscopy indicating that it stains the norepinephrine in secretory vesicles. Amperometry in conjunction with total internal reflection fluorescence (TIRF) microscopy demonstrates that the sensor can be used to observe destaining of individual chromaffin granules upon exocytosis. NS510 is the highest affinity fluorescent norepinephrine sensor currently available and can be used for measuring catecholamines in live‐cell assays.