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Perfluorocarbon‐Based 19 F MRI Nanoprobes for In Vivo Multicolor Imaging
Author(s) -
Akazawa Kazuki,
Sugihara Fuminori,
Nakamura Tatsuya,
Matsushita Hisashi,
Mukai Hiroaki,
Akimoto Rena,
Minoshima Masafumi,
Mizukami Shin,
Kikuchi Kazuya
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201810363
Subject(s) - in vivo , magnetic resonance imaging , preclinical imaging , nuclear magnetic resonance , molecular imaging , chemistry , nanotechnology , materials science , biomedical engineering , biophysics , biology , physics , medicine , radiology , microbiology and biotechnology
In vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. 19 F magnetic resonance imaging (MRI) is an emerging in vivo imaging technique because it can non‐invasively visualize 19 F nuclei without endogenous background signals. Therefore, 19 F MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon‐encapsulated silica nanoparticles that show 19 F NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct 19 F NMR peaks with sufficient sensitivity, were selected for in vivo multicolor 19 F MRI. The nanoprobes exhibited 19 F MRI signals with three colors in a living mouse. Our in vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in vivo biomolecular networks by 19 F MRI.