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Modular Redesign of a Cationic Lytic Peptide To Promote the Endosomal Escape of Biomacromolecules
Author(s) -
Azuma Yusuke,
Imai Haruka,
Kawaguchi Yoshimasa,
Nakase Ikuhiko,
Kimura Hiroshi,
Futaki Shiroh
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201807534
Subject(s) - mastoparan , endosome , peptide , chemistry , cytosol , endocytosis , intracellular , cell penetrating peptide , biophysics , biochemistry , lytic cycle , microbiology and biotechnology , cell , biology , enzyme , signal transduction , g protein , virus , virology
Endocytosis is an important route for the intracellular delivery of biomacromolecules, wherein their inefficient endosomal escape into the cytosol remains a major barrier. Based on the understanding that endosomal membranes are negatively charged, we focused on the potential of cationic lytic peptides for developing endosomolysis agents to release such entrapped molecules. As such, a venom peptide, Mastoparan X, was employed and redesigned to serve as a delivery tool. Appending a tri‐glutamate unit to the N‐terminus attenuates the cytotoxicity of Mastoparan X by about 40 fold, while introduction of a Ni II ‐dipicolylamine complex enhances cellular uptake of the peptide by about 17 fold. Using the optimized peptide, various fluorescently labeled macromolecules were successfully delivered to the cytosol, enabling live‐cell imaging of acetylated histones.