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Nanoquencher‐Based Selective Imaging of Protein Glutathionylation in Live Mammalian Cells
Author(s) -
Mao Xin,
Yuan Peiyan,
Yu Changmin,
Li Lin,
Yao Shao Q.
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201806710
Subject(s) - glutathione , endogeny , chemistry , biophysics , fluorescence , nanosensor , microbiology and biotechnology , biochemistry , nanotechnology , enzyme , biology , materials science , physics , quantum mechanics
Changes in the cellular levels of glutathione (GSH) and protein S‐glutathionylation (PSSG) are closely associated with a number of human diseases. Despite recent advances, few thiol‐reactive, small‐molecule GSH sensors could selectively detect GSH over other endogenous thiols, and none was capable of detecting PSSG in live mammalian cells. By using a dye‐loaded mesoporous silica nanoquencher (qMSN) capped with anti‐GSH antibody capable of highly selective binding toward GSH and glutathionylated proteins over other molecules, we have successfully developed a fluorescence GSH/PSSG nanosensor, which showed unprecedented selectivity toward PSSG even in the presence of GSH, had amplifiable and programmable fluorescence Turn‐ON properties, and could be used to image endogenous PSSG in live mammalian cells under stimulated conditions for the first time.