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Rationally Designed Peptidyl Virus‐Like Particles Enable Targeted Delivery of Genetic Cargo
Author(s) -
Kong Jia,
Wang Yuefei,
Zhang Jiaxing,
Qi Wei,
Su Rongxin,
He Zhimin
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201805868
Subject(s) - crispr , virus , cas9 , nanotechnology , computational biology , chemistry , dna , microbiology and biotechnology , virology , biology , biophysics , materials science , gene , biochemistry
We report a strategy to construct peptidyl virus‐like particles (pVLPs) by mimicking the human immunodeficiency virus and simian virus 40. We designed two viral peptides with cell/nucleus‐targeting capabilities that can co‐assemble in their active conformations into well‐defined nanoparticles. The self‐assembled nanoparticles can encapsulate the DNA of clustered regularly interspaced short palindromic repeat associated proteins 9 (CRISPR/Cas9) to form biodegradable pVLPs with excellent cell‐targeting ability and biocompatibility. The pVLPs can penetrate the cellular membrane and deliver genetic cargos into the nucleus through the viral entry route. The results provide a promising pathway for engineering artificial viruses with desired functions.