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Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces
Author(s) -
Kawakami Norifumi,
Kondo Hiroki,
Matsuzawa Yuki,
Hayasaka Kaoru,
Nasu Erika,
Sasahara Kenji,
Arai Ryoichi,
Miyamoto Kenji
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201805565
Subject(s) - nanoparticle , polyhedron , cationic polymerization , protein subunit , nanotechnology , chemistry , fusion protein , materials science , biochemistry , geometry , recombinant dna , polymer chemistry , mathematics , gene
Abstract Protein‐based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano‐building block for further molecular assembly.