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A Green BODIPY‐Based, Super‐Fluorogenic, Protein‐Specific Labelling Agent
Author(s) -
Chen Yingche,
Tsao Kelvin,
Acton Sydney L.,
Keillor Jeffrey W.
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201805482
Subject(s) - labelling , bodipy , peptide , lysis , chemistry , derivative (finance) , combinatorial chemistry , biochemistry , biophysics , fluorescence , biology , physics , quantum mechanics , financial economics , economics
We report the development of YC23, a novel green BODIPY‐based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.