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Cross‐Linked Aptamer–Lipid Micelles for Excellent Stability and Specificity in Target‐Cell Recognition
Author(s) -
Li Xiaowei,
Figg C. Adrian,
Wang Ruowen,
Jiang Ying,
Lyu Yifan,
Sun Hao,
Liu Yuan,
Wang Yanyue,
Teng ITing,
Hou Weijia,
Cai Ren,
Cui Cheng,
Li Long,
Pan Xiaoshu,
Sumerlin Brent S.,
Tan Weihong
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201804682
Subject(s) - aptamer , methacrylamide , micelle , chemistry , amphiphile , supramolecular chemistry , biophysics , membrane , covalent bond , combinatorial chemistry , polymerization , nanotechnology , biochemistry , copolymer , polymer , materials science , molecule , organic chemistry , biology , microbiology and biotechnology , acrylamide , aqueous solution
The specific binding ability of DNA–lipid micelles (DLMs) can be increased by the introduction of an aptamer. However, supramolecular micellar structures based on self‐assemblies of amphiphilic DLMs are expected to demonstrate low stability when interacting with cell membranes under certain conditions, which could lead to a reduction in selectivity for targeting cancer cells. We herein report a straightforward cross‐linking strategy that relies on a methacrylamide branch to link aptamer and lipid segments. By an efficient photoinduced polymerization process, covalently linked aptamer–lipid units help stabilize the micelle structure and enhance aptamer probe stability, further improving the targeting ability of the resulting nanoassembly. Besides the development of a facile cross‐linking method, this study clarifies the relationship between aptamer–lipid concentration and the corresponding binding ability.