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Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC‐MS
Author(s) -
Zhu Ying,
Clair Geremy,
Chrisler William B.,
Shen Yufeng,
Zhao Rui,
Shukla Anil K.,
Moore Ronald J.,
Misra Ravi S.,
Pryhuber Gloria S.,
Smith Richard D.,
Ansong Charles,
Kelly Ryan T.
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201802843
Subject(s) - proteome , proteomics , chemistry , single cell analysis , cell sorting , hela , cell , mesenchymal stem cell , microfluidics , sample preparation , microbiology and biotechnology , fluorescence , nanotechnology , biophysics , biochemistry , biology , chromatography , materials science , physics , quantum mechanics , gene
We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence‐activated cell sorting was employed to deposit cells into a newly developed nanodroplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC‐MS. An average of circa 670 protein groups were confidently identified from single HeLa cells, which is a far greater level of proteome coverage for single cells than has been previously reported. We demonstrate that the single‐cell proteomics platform can be used to differentiate cell types from enzyme‐dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.