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Valency‐Controlled Framework Nucleic Acid Signal Amplifiers
Author(s) -
Liu Qi,
Ge Zhilei,
Mao Xiuhai,
Zhou Guobao,
Zuo Xiaolei,
Shen Juwen,
Shi Jiye,
Li Jiang,
Wang Lihua,
Chen Xiaoqing,
Fan Chunhai
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201802701
Subject(s) - nucleic acid , biosensor , biomolecule , amplifier , chemistry , nanotechnology , biophysics , materials science , biology , biochemistry , optoelectronics , cmos
Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in in vitro biomimetic systems generally lack the spatiotemporal regulation in vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy to develop valence‐controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence‐controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor‐relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3–5 orders of magnitude and improved dynamic range.