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Complexing DNA Origami Frameworks through Sequential Self‐Assembly Based on Directed Docking
Author(s) -
Suzuki Yuki,
Sugiyama Hiroshi,
Endo Masayuki
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201801983
Subject(s) - dna origami , supramolecular chemistry , nanotechnology , dna nanotechnology , docking (animal) , self assembly , dna , nanoscopic scale , atomic force microscopy , chemistry , lipid bilayer , materials science , membrane , crystallography , biophysics , nanostructure , crystal structure , biology , biochemistry , medicine , nursing
Ordered DNA origami arrays have the potential to compartmentalize space into distinct periodic domains that can incorporate a variety of nanoscale objects. Herein, we used the cavities of a preassembled 2D DNA origami framework to incorporate square‐shaped DNA origami structures (SQ‐origamis). The framework was self‐assembled on a lipid bilayer membrane from cross‐shaped DNA origami structures (CR‐origamis) and subsequently exposed to the SQ‐origamis. High‐speed AFM revealed the dynamic adsorption/desorption behavior of the SQ‐origamis, which resulted in continuous changing of their arrangements in the framework. These dynamic SQ‐origamis were trapped in the cavities by increasing the Mg 2+ concentration or by introducing sticky‐ended cohesions between extended staples, both from the SQ‐ and CR‐origamis, which enabled the directed docking of the SQ‐origamis. Our study offers a platform to create supramolecular structures or systems consisting of multiple DNA origami components.