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Controllable Assembly of Enzymes for Multiplexed Lab‐on‐a‐Chip Bioassays with a Tunable Detection Range
Author(s) -
Xianyu Yunlei,
Wu Jing,
Chen Yiping,
Zheng Wenshu,
Xie Mengxia,
Jiang Xingyu
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201801815
Subject(s) - detection limit , immunoassay , multiplexing , bioassay , horseradish peroxidase , chemistry , chromatography , biosensor , lab on a chip , nanotechnology , multiplex , microfluidics , enzyme , materials science , computer science , biochemistry , bioinformatics , antibody , biology , telecommunications , genetics , immunology
Abstract Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab‐on‐a‐chip immunoassays with a tunable detection range from pg mL −1 to μg mL −1 . Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab‐on‐a‐chip format, with a limit of detection of 0.47 pg mL −1 for interleukin‐6, 2.6 pg mL −1 for procalcitonin, and 40 ng mL −1 for C‐reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low‐abundance and high‐abundance biomarkers with a broad detection range, which holds great promise as a point‐of‐care platform for biomedical diagnostics.