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Super‐Chelators for Advanced Protein Labeling in Living Cells
Author(s) -
Gatterdam Karl,
Joest Eike F.,
Dietz Marina S.,
Heilemann Mike,
Tampé Robert
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201800827
Subject(s) - nitrilotriacetic acid , chemistry , small molecule , chelation , biophysics , protein tag , super resolution microscopy , molecule , combinatorial chemistry , nanotechnology , fluorescence , fluorescence microscope , biochemistry , fusion protein , biology , materials science , recombinant dna , physics , organic chemistry , quantum mechanics , gene
Live‐cell labeling, super‐resolution microscopy, single‐molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of protein function is essential to derive unbiased insights into cellular processes. Herein, we define a new class of hexavalent N ‐nitrilotriacetic acid ( hexa NTA) chelators, displaying the highest affinity and stability of all NTA‐based small interaction pairs described so far. Coupled to bright organic fluorophores with fine‐tuned photophysical properties, the super‐chelator probes were delivered into human cells by chemically gated nanopores. These super‐chelators permit kinetic profiling, multiplexed labeling of His 6 ‐ and His 12 ‐tagged proteins as well as single‐molecule‐based super‐resolution imaging.