Premium
A Multi‐signal Fluorescent Probe with Multiple Binding Sites for Simultaneous Sensing of Cysteine, Homocysteine, and Glutathione
Author(s) -
Yin Guoxing,
Niu Tingting,
Gan Yabing,
Yu Ting,
Yin Peng,
Chen Haimin,
Zhang Youyu,
Li Haitao,
Yao Shouzhuo
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201800485
Subject(s) - glutathione , cysteine , fluorescence , coumarin , chemistry , homocysteine , excitation wavelength , endogeny , photochemistry , biophysics , biochemistry , enzyme , organic chemistry , biology , physics , quantum mechanics
A novel fluorescent probe was developed by integrating chlorinated coumarin and benzothiazolylacetonitrile and exploited for simultaneous detection of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). Featuring four binding sites and different reaction mechanisms for different biothiols, this probe exhibited rapid fluorescence turn‐on for distinguishing Cys, Hcy, and GSH with 108‐, 128‐, 30‐fold fluorescence increases at 457, 559, 529 nm, respectively, across different excitation wavelengths. Furthermore, the probe was successfully applied to the fluorescence imaging of endogenous Cys and GSH and exogenous Cys, Hcy, and GSH in living cells.