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Miniaturized Biosensors to Preserve and Monitor Cultural Heritage: from Medical to Conservation Diagnosis
Author(s) -
Sciutto Giorgia,
Zangheri Martina,
Anfossi Laura,
Guardigli Massimo,
Prati Silvia,
Mirasoli Mara,
Di Nardo Fabio,
Baggiani Claudio,
Mazzeo Rocco,
Roda Aldo
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201713298
Subject(s) - cultural heritage , nanotechnology , computer science , point of care testing , chemiluminescence , biosensor , bioanalysis , point (geometry) , systems engineering , process engineering , engineering , materials science , chemistry , chromatography , medicine , archaeology , geography , immunology , geometry , mathematics
The point‐of‐care testing concept has been exploited to design and develop portable and cheap bioanalytical systems that can be used on‐site by conservators. These systems employ lateral flow immunoassays to simultaneously detect two proteins (ovalbumin and collagen) in artworks. For an in‐depth study on the application of these portable biosensors, both chemiluminescent and colorimetric detections were developed and compared in terms of sensitivity and feasibility. The chemiluminescent system displayed the best analytical performance (that is, two orders of magnitude lower limits of detection than the colorimetric system). To simplify its use, a disposable cartridge was designed ad hoc for this specific application. These results highlight the enormous potential of these inexpensive, easy‐to‐use, and minimally invasive diagnostic tools for conservators in the cultural heritage field.