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Optogenetic Control of Voltage‐Gated Calcium Channels
Author(s) -
Ma Guolin,
Liu Jindou,
Ke Yuepeng,
Liu Xin,
Li Minyong,
Wang Fen,
Han Gang,
Huang Yun,
Wang Youjun,
Zhou Yubin
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201713080
Subject(s) - optogenetics , depolarization , chemistry , biophysics , voltage dependent calcium channel , gtpase , calcium signaling , calcium , microbiology and biotechnology , neuroscience , biology , biochemistry , organic chemistry
Voltage‐gated Ca 2+ (Ca V ) channels mediate Ca 2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca V channel modulators, to enable photo‐tunable modulation of Ca V channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca V channels could find broad applications in interrogating a wide range of Ca V ‐mediated physiological processes.