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Hybrid Indicators for Fast and Sensitive Voltage Imaging
Author(s) -
Xu Yongxian,
Peng Luxin,
Wang Sicong,
Wang Anqi,
Ma Ruirui,
Zhou Ying,
Yang Jiahe,
Sun Deen,
Lin Wei,
Chen Xing,
Zou Peng
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201712614
Subject(s) - rhodopsin , voltage , fluorescence , materials science , nanotechnology , coupling (piping) , optoelectronics , membrane , chemistry , biophysics , optics , biology , physics , biochemistry , retinal , quantum mechanics , metallurgy
Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. A fluorescent voltage indicator is presented that enables the investigation of electrical signaling at high spatial resolution. The method is built upon the site‐specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid indicator scaffold that is one of the most sensitive and fastest orange‐colored voltage indicators developed to date. We applied this technique to optically map electrical connectivity in cultured cells, which revealed gap junction‐mediated long‐range coupling that spanned over hundreds of micrometers.