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Optical Control of a Biological Reaction–Diffusion System
Author(s) -
Glock Philipp,
Broichhagen Johannes,
Kretschmer Simon,
Blumhardt Philipp,
Mücksch Jonas,
Trauner Dirk,
Schwille Petra
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201712002
Subject(s) - photoswitch , peptide , chemistry , diffusion , azobenzene , biophysics , membrane , combinatorial chemistry , covalent bond , nanotechnology , biological system , materials science , molecule , biochemistry , photochemistry , organic chemistry , physics , biology , thermodynamics
Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction–diffusion (R–D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R–D system consisting of the antagonistic proteins MinD and MinE. A MinE‐derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide‐mediated depletion of MinD from the membrane. In addition to providing an on–off switch for pattern formation, we achieve frequency‐locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.