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A Crosslinked Nucleic Acid Nanogel for Effective siRNA Delivery and Antitumor Therapy
Author(s) -
Ding Fei,
Mou Quanbing,
Ma Yuan,
Pan Gaifang,
Guo Yuanyuan,
Tong Gangsheng,
Choi Chung Hang Jonathan,
Zhu Xinyuan,
Zhang Chuan
Publication year - 2018
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201711242
Subject(s) - small interfering rna , nucleic acid , nanogel , gene silencing , chemistry , polycaprolactone , transfection , in vivo , biophysics , dna , rna , rna interference , nanocarriers , in vitro , nanotechnology , materials science , drug delivery , biochemistry , polymer , biology , gene , organic chemistry , microbiology and biotechnology
Abstract Functional siRNAs are employed as cross‐linkers to direct the self‐assembly of DNA‐grafted polycaprolactone (DNA‐ g ‐PCL) brushes to form spherical and nanosized hydrogels via nucleic acid hybridization in which small interfering RNAs (siRNAs) are fully embedded and protected for systemic delivery. Owing to the existence of multivalent mutual crosslinking events inside, the crosslinked nanogels with tunable size exhibit not only good thermostability, but also remarkable physiological stability that can resist the enzymatic degradation. As a novel siRNA delivery system with spherical nucleic acid (SNA) architecture, the crosslinked nanogels can assist the delivery of siRNAs into different cells without any transfection agents and achieve the gene silencing effectively both in vitro and in vivo, through which a significant inhibition of tumor growth is realized in the anticancer treatment.