Premium
Breaking the Limits in Analyzing Carbohydrate Recognition by NMR Spectroscopy: Resolving Branch‐Selective Interaction of a Tetra‐Antennary N ‐Glycan with Lectins
Author(s) -
Canales Angeles,
Boos Irene,
Perkams Lukas,
Karst Lukas,
Luber Thomas,
Karagiannis Theodoros,
Domínguez Gemma,
Cañada F. Javier,
PérezCastells Javier,
Häussinger Daniel,
Unverzagt Carlo,
JiménezBarbero Jesus
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201709130
Subject(s) - glycan , chemistry , nuclear magnetic resonance spectroscopy , datura stramonium , lectin , stereochemistry , molecular recognition , biochemistry , glycoprotein , molecule , organic chemistry , biology , horticulture
The biological recognition of complex‐type N ‐glycans is part of many key physiological and pathological events. Despite their importance, the structural characterization of these events remains unsolved. The inherent flexibility of N ‐glycans hampers crystallization and the chemical equivalence of individual branches precludes their NMR characterization. By using a chemoenzymatically synthesized tetra‐antennary N ‐glycan conjugated to a lanthanide binding tag, the NMR signals under paramagnetic conditions discriminated all four N ‐acetyl lactosamine antennae with unprecedented resolution. The NMR data revealed the conformation of the N ‐glycan and permitted for the first time the direct identification of individual branches involved in the recognition by two N ‐acetyllactosamine‐binding lectins, Datura stramonium seed lectin (DSL) and Ricinus Communis agglutinin (RCA120).