Premium
A Thermophilic Tetramolecular G‐Quadruplex/Hemin DNAzyme
Author(s) -
Guo Yuehua,
Chen Jielin,
Cheng Mingpan,
Monchaud David,
Zhou Jun,
Ju Huangxian
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201708964
Subject(s) - deoxyribozyme , hemin , catalysis , chemistry , abts , g quadruplex , thermophile , oxidizing agent , combinatorial chemistry , peroxidase , nanomaterial based catalyst , enzyme , biochemistry , organic chemistry , dna , antioxidant , heme , dpph
The quadruplex‐based DNAzyme system is one of the most useful artificial enzymes or catalysts; their unique properties make them reliable alternatives to proteins for performing catalytic transformation. The first prototype of a thermally stable DNAzyme system is presented. This thermophilic DNAzyme is capable of oxidizing substrates at high temperatures (up to 95 °C) and long reaction times (up to 18 h at 75 °C). The catalytic activity of the DNAzymes were investigated with the standard peroxidase‐mimicking oxidation of 2,2′‐azino‐bis(3‐ethylbenzothiozoline‐6‐sulfonic acid) (ABTS) by H 2 O 2 . The step‐by‐step design of this unique heat‐activated G‐quadruplex/hemin catalyst, including the modification of adenines at both ends of G‐tracts, the choice of cation, and its concentration for DNAzyme stabilization, is described. This work investigates thoroughly the molecular basis of these catalytic properties and provides an example of an industrially relevant application.