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A Peroxisome‐Inspired Chemiluminescent Silica Nanodevice for the Intracellular Detection of Biomarkers and Its Application to Insulin‐Sensitizer Screening
Author(s) -
Jie Xu,
Yang Haimei,
Wang Min,
Zhang Yue,
Wei Weili,
Xia Zhining
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201708958
Subject(s) - chemiluminescence , nanodevice , peroxisome , chemistry , intracellular , uric acid , biochemistry , oxidase test , peroxisome proliferator , nadph oxidase , metabolomics , combinatorial chemistry , enzyme , nanotechnology , chromatography , materials science , receptor
The profiling of oxidase‐catalyzed biomarkers is an essential procedure for the diagnosis and precise treatment of metabolic diseases. Inspired by the metabolism of H 2 O 2 in peroxisomes, a novel chemiluminescent silica nanodevice (CSN) was designed for the sensitive and selective sensing of intracellular oxidase‐catalyzed biomarkers. Oxidases catalyzed the oxidation of biomarkers followed by the production of H 2 O 2 , and then the generated H 2 O 2 was employed to trigger chemiluminescence of the CSN. Utilizing this nanodevice, we not only accurately quantified intracellular glucose but also developed its further application for facile insulin sensitizer screening. Furthermore, sensitive and multiparametric analysis of oxidase‐catalyzed biomarkers like lactic acid, uric acid, and ethanol was demonstrated. Thus, this peroxisome‐inspired CSN holds great promise for the general diagnosis of metabolic diseases and in drug discovery.