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An Epitope‐Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell–Biomaterial Interactions
Author(s) -
Pan Guoqing,
Shinde Sudhirkumar,
Yeung Sing Yee,
Jakštaitė Miglė,
Li Qianjin,
Wingren Anette Gjörloff,
Sellergren Börje
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201708635
Subject(s) - biointerface , epitope , chemistry , imprinting (psychology) , biophysics , molecular imprinting , peptide , molecular recognition , cell adhesion , nanotechnology , materials science , cell , biochemistry , selectivity , antigen , molecule , biology , genetics , organic chemistry , gene , catalysis
In this study, an epitope‐imprinting strategy was employed for the dynamic display of bioactive ligands on a material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope‐tagged cell‐adhesive peptide ligand (RGD: Arg‐Gly‐Asp). Owing to reversible epitope‐binding affinity, ligand presentation and thereby cell adhesion could be controlled. As compared to current strategies for the fabrication of dynamic biointerfaces, for example, through reversible covalent or host–guest interactions, such a molecularly tunable dynamic system based on a surface‐imprinting process may unlock new applications in in situ cell biology, diagnostics, and regenerative medicine.