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Biological Imaging with Medium‐Sensitive Bichromatic Flexible Fluorescent Dyes
Author(s) -
Cao Yang,
Wang Xiaozhu,
Shi Xiaolei,
Clee Susanne M.,
McGeer Patrick L.,
Wolf Michael O.,
Orvig Chris
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201708529
Subject(s) - fluorescence , fluorophore , chemistry , photochemistry , molecule , microscopy , lipid droplet , fluorescence microscope , biophysics , nanotechnology , materials science , organic chemistry , optics , biochemistry , physics , biology
A family of environment‐sensitive shape‐shifting molecules have been developed as flexible fluorescent (FlexFluor) dyes for biological imaging applications. These compounds feature a flexible bithiophene‐based fluorophore that gives rise to different emission colors in lipophilic or hydrophilic environments, as well as side groups that can be synthetically modified with ease. FlexFluor dyes are the first fluorescent dyes in which emission color can be used to indicate lipid/water environments. The behavior of these dyes in different solvents was studied, and used to simultaneously highlight lipid and water contents in adipose and brain tissues using optical fluorescence microscopy.