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Peptide Logic Circuits Based on Chemoenzymatic Ligation for Programmable Cell Apoptosis
Author(s) -
Li Yong,
Sun Sujuan,
Fan Lin,
Hu Shanfang,
Huang Yan,
Zhang Ke,
Nie Zhou,
Yao Shouzhou
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201708327
Subject(s) - peptide , logic gate , modular design , chemistry , computer science , microbiology and biotechnology , biology , biochemistry , algorithm , operating system
Abstract A novel and versatile peptide‐based bio‐logic system capable of regulating cell function is developed using sortase A (SrtA), a peptide ligation enzyme, as a generic processor. By modular peptide design, we demonstrate that mammalian cells apoptosis can be programmed by peptide‐based logic operations, including binary and combination gates (AND, INHIBIT, OR, and AND‐INHIBIT), and a complex sequential logic circuit (multi‐input keypad lock). Moreover, a proof‐of‐concept peptide regulatory circuit was developed to analyze the expression profile of cell‐secreted protein biomarkers and trigger cancer‐cell‐specific apoptosis.