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Rational Design of a Robust Fluorescent Probe for the Detection of Endogenous Carbon Monoxide in Living Zebrafish Embryos and Mouse Tissue
Author(s) -
Liu Keyin,
Kong Xiuqi,
Ma Yanyan,
Lin Weiying
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201707518
Subject(s) - fluorescence , carbon monoxide , endogeny , zebrafish , chemistry , rational design , in vivo , biophysics , photochemistry , nanotechnology , biology , materials science , biochemistry , physics , microbiology and biotechnology , gene , catalysis , quantum mechanics
Carbon monoxide (CO) is one of the most important gaseous signal molecules in biological systems. However, the investigation of the functions of CO in living organisms is restricted by the lack of functional molecular tools. To address this critical challenge, we present herein the rational design, synthesis, and in vivo imaging studies of a powerful two‐photon excited near‐infrared fluorescent probe ( 1‐Ac ) for endogenous CO monitoring. The advantageous features of the new probe include high stability, low background fluorescence, large fluorescence enhancement, high sensitivity, and two‐photon excitation with emission in the near‐infrared region. Significantly, these merits of the probe enable the tracking of endogenous CO in zebrafish embryos and mouse tissues for the first time.