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A Polymer/Peptide Complex‐Based Sensor Array That Discriminates Bacteria in Urine
Author(s) -
Han Jinsong,
Cheng Haoran,
Wang Benhua,
Braun Markus Santhosh,
Fan Xiaobo,
Bender Markus,
Huang Wei,
Domhan Cornelius,
Mier Walter,
Lindner Thomas,
Seehafer Kai,
Wink Michael,
Bunz Uwe H. F.
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201706101
Subject(s) - bacteria , fluorescence , peptide , urine , chemistry , strain (injury) , antimicrobial , linear discriminant analysis , biophysics , chromatography , combinatorial chemistry , biology , biochemistry , genetics , organic chemistry , anatomy , physics , statistics , mathematics , quantum mechanics
A negatively charged poly( para ‐phenyleneethynylene) (PPE) forms electrostatic complexes with four positively charged antimicrobial peptides (AMP). The AMPs partially quench the fluorescence of the PPE and discriminate fourteen different bacteria in water and in human urine by pattern‐based fluorescence recognition; the AMP‐PPE complexes bind differentially to the components of bacterial surfaces. The bacterial species and strains form clusters according to staining properties (Gram‐positive and Gram‐negative) or genetic similarity (genus, species, and strain). The identification and data treatment is performed by pattern evaluation with linear discriminant analysis (LDA) of the collected fluorescence intensity data.