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An Electrochemical Biosensor with Dual Signal Outputs: Toward Simultaneous Quantification of pH and O 2 in the Brain upon Ischemia and in a Tumor during Cancer Starvation Therapy
Author(s) -
Liu Li,
Zhao Fan,
Liu Wei,
Zhu Tong,
Zhang John Z. H.,
Chen Chen,
Dai Zhihui,
Peng Huisheng,
Huang JunLong,
Hu Qin,
Bu Wenbo,
Tian Yang
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201705615
Subject(s) - hemin , biosensor , chemistry , electrochemistry , selectivity , signal (programming language) , cancer therapy , molecule , analytical chemistry (journal) , combinatorial chemistry , chromatography , electrode , cancer , biochemistry , enzyme , organic chemistry , computer science , heme , catalysis , medicine , programming language
Herein, we develop a novel method for designing electrochemical biosensors with both current and potential signal outputs for the simultaneous determination of two species in a living system. Oxygen (O 2 ) and pH, simple and very important species, are employed as model molecules. By designing and synthesizing a new molecule, Hemin‐aminoferrocene (Hemin‐Fc), we create a single electrochemical biosensor for simultaneous detection and ratiometric quantification of O 2 and pH in the brain. The reduction peak current of the hemin group increases with the concentration of O 2 from 1.3 to 200.6 μ m . Meanwhile, the peak potential positively shifts with decreasing pH from 8.0 to 5.5, resulting in the simultaneous determination of O 2 and pH. The Fc group can serve as an internal reference for ratiometric biosensing because its current and potential signals remain almost constant with variations of O 2 and pH. The developed biosensor has high temporal and spatial resolutions, as well as remarkable selectivity and accuracy, and is successfully applied in the real‐time quantification of O 2 and pH in the brain upon ischemia, as well as in tumor during cancer therapy.