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Bioinspired, Size‐Tunable Self‐Assembly of Polymer–Lipid Bilayer Nanodiscs
Author(s) -
Ravula Thirupathi,
Ramadugu Sudheer Kumar,
Di Mauro Giacomo,
Ramamoorthy Ayyalusamy
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201705569
Subject(s) - nanodisc , polymer , lipid bilayer , self assembly , bilayer , model lipid bilayer , chemistry , membrane , biomolecule , materials science , nanotechnology , chemical engineering , lipid bilayer phase behavior , organic chemistry , engineering , biochemistry
Polymer‐based nanodiscs are valuable tools in biomedical research that can offer a detergent‐free solubilization of membrane proteins maintaining their native lipid environment. Herein, we introduce a novel ca. 1.6 kDa SMA‐based polymer with styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are useful to reconstitute membrane proteins for structural and functional studies. The physicochemical properties and the mechanism of formation of polymer‐based nanodiscs are characterized by light scattering, NMR, FT‐IR, and TEM. A remarkable feature is that nanodiscs of different sizes, from nanometer to sub‐micrometer diameter, can be produced by varying the lipid‐to‐polymer ratio. The small‐size nanodiscs (up to ca. 30 nm diameter) can be used for solution NMR spectroscopy studies whereas the magnetic‐alignment of macro‐nanodiscs (diameter of > ca. 40 nm) can be exploited for solid‐state NMR studies on membrane proteins.