Premium
Global Mapping of Protein–Lipid Interactions by Using Modified Choline‐Containing Phospholipids Metabolically Synthesized in Live Cells
Author(s) -
Wang Danyang,
Du Shubo,
CazenaveGassiot Amaury,
Ge Jingyan,
Lee JunSeok,
Wenk Markus R.,
Yao Shao Q.
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201702509
Subject(s) - phospholipid , biochemistry , choline , chemistry , proteomics , phospholipid transfer protein , protein–protein interaction , membrane , gene
Abstract The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne‐containing choline head group and a diazirine‐modified fatty acid simultaneously into choline‐containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid‐crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid‐interacting proteins, some of which were further validated.