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In‐Phase Ultra High‐Resolution In Vivo NMR
Author(s) -
Fugariu Ioana,
Bermel Wolfgang,
Lane Daniel,
Soong Ronald,
Simpson Andre J.
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201701097
Subject(s) - nmr spectra database , nuclear magnetic resonance , phase (matter) , chemical shift , isotropy , chemical physics , nuclear magnetic resonance spectroscopy , in vivo , resolution (logic) , spectral line , intermolecular force , relaxation (psychology) , biomolecule , chemistry , nanotechnology , materials science , physics , molecule , optics , computer science , biology , microbiology and biotechnology , organic chemistry , astronomy , artificial intelligence , neuroscience
Although current NMR techniques allow organisms to be studied in vivo, magnetic susceptibility distortions, which arise from inhomogeneous distributions of chemical moieties, prevent the acquisition of high‐resolution NMR spectra. Intermolecular single quantum coherence (iSQC) is a technique that breaks the sample's spatial isotropy to form long range dipolar couplings, which can be exploited to extract chemical shift information free of perturbations. While this approach holds vast potential, present practical limitations include radiation damping, relaxation losses, and non‐phase sensitive data. Herein, these drawbacks are addressed, and a new technique termed in‐phase iSQC (IP‐iSQC) is introduced. When applied to a living system, high‐resolution NMR spectra, nearly identical to a buffer extract, are obtained. The ability to look inside an organism and extract a high‐resolution metabolic profile is profound and should find applications in fields in which metabolism or in vivo processes are of interest.