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Efficient Gene Transfection through Inhibition of β‐Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
Author(s) -
Jana Poulami,
Samanta Krishnananda,
Bäcker Sandra,
Zellermann Elio,
Knauer Shirley,
Schmuck Carsten
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201700713
Subject(s) - colloidal gold , peptide , gene delivery , amphiphile , transfection , biophysics , chemistry , nanoparticle , beta sheet , cationic polymerization , fibril , nanotechnology , biochemistry , materials science , copolymer , polymer , gene , polymer chemistry , organic chemistry , biology
The effect of citrate‐stabilized gold nanoparticles (AuNPs) on the secondary structure of an artificial β‐sheet‐forming cationic peptide has been studied. The AuNPs inhibited β‐sheet formation and led to fragmented fibrils and spherical oligomers with assembled AuNPs on their surface. Besides this structural change, the functional properties of the peptide are also different. Whereas the peptide was unable to act as a vector for gene delivery, formation of a complex with AuNPs allowed successful gene delivery into cells.