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An Exonuclease III‐Powered, On‐Particle Stochastic DNA Walker
Author(s) -
Qu Xiangmeng,
Zhu Dan,
Yao Guangbao,
Su Shao,
Chao Jie,
Liu Huajie,
Zuo Xiaolei,
Wang Lihua,
Shi Jiye,
Wang Lianhui,
Huang Wei,
Pei Hao,
Fan Chunhai
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201611777
Subject(s) - exonuclease iii , dna , biophysics , dna nanotechnology , exonuclease , chemistry , nanotechnology , total internal reflection fluorescence microscope , a dna , biological system , fluorescence , physics , biology , biochemistry , materials science , optics , polymerase , escherichia coli , gene
DNA‐based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonuclease III (Exo III)‐powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)‐based 3D track. The motion is propelled by unidirectional Exo III digestion of hybridized DNA tracks in a burnt‐bridge mechanism. The operation of this Exo III‐propelled DNA walker was monitored in real time and at the single‐particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA‐based stochastic DNA walker by exploiting movement‐triggered cascade signal amplification.