Premium
Conformational SERS Classification of K‐Ras Point Mutations for Cancer Diagnostics
Author(s) -
MorlaFolch Judit,
GisbertQuilis Patricia,
Masetti Matteo,
GarciaRico Eduardo,
AlvarezPuebla Ramon A.,
Guerrini Luca
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201611243
Subject(s) - point mutation , nucleobase , molecular diagnostics , chemometrics , computational biology , cancer , colorectal cancer , mutation , gene , chemistry , genetics , biology , dna , chromatography
Point mutations in Ras oncogenes are routinely screened for diagnostics and treatment of tumors (especially in colorectal cancer). Here, we develop an optical approach based on direct SERS coupled with chemometrics for the study of the specific conformations that single‐point mutations impose on a relatively large fragment of the K‐Ras gene (141 nucleobases). Results obtained offer the unambiguous classification of different mutations providing a potentially useful insight for diagnostics and treatment of cancer in a sensitive, fast, direct and inexpensive manner.