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Capturing Excited States in the Fast‐Intermediate Exchange Limit in Biological Systems Using 1 H NMR Spectroscopy
Author(s) -
Steiner Emilie,
Schlagnitweit Judith,
Lundström Patrik,
Petzold Katja
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201609102
Subject(s) - excited state , biomolecule , characterization (materials science) , spectroscopy , relaxation (psychology) , chemistry , nuclear magnetic resonance spectroscopy , chemical physics , dispersion (optics) , atomic physics , physics , nanotechnology , materials science , stereochemistry , quantum mechanics , psychology , social psychology , biochemistry
Abstract Changes in molecular structure are essential for the function of biomolecules. Characterization of these structural fluctuations can illuminate alternative states and help in correlating structure to function. NMR relaxation dispersion (RD) is currently the only method for detecting these alternative, high‐energy states. In this study, we present a versatile 1 H R 1ρ RD experiment that not only extends the exchange timescales at least three times beyond the rate limits of 13 C/ 15 N R 1ρ and ten times for CPMG experiments, but also makes use of easily accessible probes, thus allowing a general description of biologically important excited states. This technique can be used to extract chemical shifts for the structural characterization of excited states and to elucidate complex excited states.