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NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
Author(s) -
RuizGonzález Rubén,
BresolíObach Roger,
Gulías Òscar,
Agut Montserrat,
Savoie Huguette,
Boyle Ross W.,
ll Santi,
Giuntini Francesca
Publication year - 2017
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201609050
Subject(s) - nanoprobe , singlet oxygen , fluorescence , covalent bond , chemistry , nanoparticle , photochemistry , bovine serum albumin , oxygen , nanotechnology , biophysics , materials science , biochemistry , optics , organic chemistry , physics , biology
A biocompatible fluorescent nanoprobe for singlet oxygen ( 1 O 2 ) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green ® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1 O 2 . In contrast to its molecular counterpart, the optimum SOSG‐based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1 O 2 with an increase in fluorescence.