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3D Framework DNA Origami with Layered Crossovers
Author(s) -
Hong Fan,
Jiang Shuoxing,
Wang Tong,
Liu Yan,
Yan Hao
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201607050
Subject(s) - dna origami , parallelogram , nanotechnology , nanostructure , dna , dna nanotechnology , crossover , materials science , layer (electronics) , nanoscopic scale , design strategy , computer science , chemistry , engineering , hinge , mechanical engineering , biochemistry , artificial intelligence
Designer DNA architectures with nanoscale geometric controls provide a programmable molecular toolbox for engineering complex nanodevices. Scaffolded DNA origami has dramatically improved our ability to design and construct DNA nanostructures with finite size and spatial addressability. Here we report a novel design strategy to engineer multilayered wireframe DNA structures by introducing crossover pairs that connect neighboring layers of DNA double helices. These layered crossovers (LX) allow the scaffold or helper strands to travel through different layers and can control the relative orientation of DNA helices in neighboring layers. Using this design strategy, we successfully constructed four versions of two‐layer parallelogram structures with well‐defined interlayer angles, a three‐layer structure with triangular cavities, and a 9‐ and 15‐layer square lattices. This strategy provides a general route to engineer 3D framework DNA nanostructures with controlled cavities and opportunities to design host–guest networks analogs to those produced with metal organic frameworks.