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Profiling Metal Oxides with Lipids: Magnetic Liposomal Nanoparticles Displaying DNA and Proteins
Author(s) -
Wang Feng,
Zhang Xiaohan,
Liu Yibo,
Lin Zhi Yuan William,
Liu Biwu,
Liu Juewen
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201606603
Subject(s) - liposome , calcein , lipid bilayer , chemistry , nanoparticle , biomolecule , biosensor , membrane , nanomedicine , nanotechnology , chemical engineering , materials science , biochemistry , engineering
Metal oxides include many important materials with various surface properties. For biomedical and analytical applications, it is desirable to engineer their biocompatible interfaces. Herein, a phosphocholine liposome (DOPC) and its headgroup dipole flipped counterpart (DOCP) were mixed with ten common oxides. Using the calcein leakage assay, cryo‐TEM, and ζ‐potential measurement, these oxides were grouped into three types. The type 1 oxides (Fe 3 O 4 , TiO 2 , ZrO 2 , Y 2 O 3 , ITO, In 2 O 3 , and Mn 2 O 3 ) form supported bilayers only with DOCP. Type 2 (SiO 2 ) forms supported bilayers only with DOPC; type 3 (ZnO and NiO) are cationic and damage lipid membranes. Magnetic Fe 3 O 4 nanoparticles were further studied for conjugation of fluorophores, proteins, and DNA to the supported DOCP bilayers via lipid headgroup labeling, covalent linking, or lipid insertion. Delivery of the conjugates to cells and selective DNA hybridization were demonstrated. This work provides a general solution for coating the type 1 oxides with a simple mixing in water, facilitating applications in biosensing, separation, and nanomedicine.