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Enhancing the Sensitivity of CPMG Relaxation Dispersion to Conformational Exchange Processes by Multiple‐Quantum Spectroscopy
Author(s) -
Yuwen Tairan,
Vallurupalli Pramodh,
Kay Lewis E.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201605843
Subject(s) - dispersion (optics) , chemistry , relaxation (psychology) , conformational isomerism , protonation , spectroscopy , nuclear magnetic resonance spectroscopy , quantum chemical , deuterium , chemical physics , nuclear magnetic resonance , molecular physics , molecule , atomic physics , stereochemistry , physics , optics , quantum mechanics , organic chemistry , ion , psychology , social psychology
A triple‐quantum 1 H Carr–Purcell–Meiboom–Gill NMR relaxation dispersion experiment is presented that uses methyl group probes as reporters of conformational exchange in highly deuterated, methyl‐protonated proteins. Significantly larger dispersion profiles, by as much as a factor of nine, can be obtained relative to single‐quantum approaches, thus offering very significant advantages in applications involving interconverting conformers with only small changes in structure or in studies of rare states that are at very low populations. Applications to a number of protein systems are presented where the utility of the method, including its improved sensitivity to chemical exchange processes, is established.