Premium
An Epigenetics‐Inspired DNA‐Based Data Storage System
Author(s) -
Mayer Clemens,
McInroy Gordon R.,
Murat Pierre,
Van Delft Pieter,
Balasubramanian Shankar
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201605531
Subject(s) - dna , cytosine , interlacing , deamination , chemistry , nanotechnology , computer science , computational biology , combinatorial chemistry , biological system , biology , materials science , biochemistry , enzyme , operating system
Abstract Biopolymers are an attractive alternative to store and circulate information. DNA, for example, combines remarkable longevity with high data storage densities and has been demonstrated as a means for preserving digital information. Inspired by the dynamic, biological regulation of (epi)genetic information, we herein present how binary data can undergo controlled changes when encoded in synthetic DNA strands. By exploiting differential kinetics of hydrolytic deamination reactions of cytosine and its naturally occurring derivatives, we demonstrate how multiple layers of information can be stored in a single DNA template. Moreover, we show that controlled redox reactions allow for interconversion of these DNA‐encoded layers of information. Overall, such interlacing of multiple messages on synthetic DNA libraries showcases the potential of chemical reactions to manipulate digital information on (bio)polymers.