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Upregulation of an Artificial Zymogen by Proteolysis
Author(s) -
Liu Zhe,
Lebrun Vincent,
Kitanosono Taku,
Mallin Hendrik,
Köhler Valentin,
Häussinger Daniel,
Hilvert Donald,
Kobayashi Shu,
Ward Thomas R.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201605010
Subject(s) - zymogen , proteolysis , downregulation and upregulation , protease , hydrogenase , enzyme , chemistry , biochemistry , gene
Regulation of enzymatic activity is vital to living organisms. Here, we report the development and the genetic optimization of an artificial zymogen requiring the action of a natural protease to upregulate its latent asymmetric transfer hydrogenase activity.