Premium
Immobilization of the Gas Signaling Molecule H 2 S by Radioisotopes: Detection, Quantification, and In Vivo Imaging
Author(s) -
Sarkar Swarbhanu,
Ha Yeong Su,
Soni Nisarg,
An Gwang Il,
Lee Woonghee,
Kim Min Hwan,
Huynh Phuong Tu,
Ahn Heesu,
Bhatt Nikunj,
Lee Yong Jin,
Kim Jung Young,
Park Kwon Moo,
Ishii Isao,
Kang ShinGeol,
Yoo Jeongsoo
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201603813
Subject(s) - in vivo , chemistry , hydrogen sulfide , positron emission tomography , molecule , molecular imaging , preclinical imaging , pet imaging , radiochemistry , cyclen , detection limit , luminescence , nuclear medicine , materials science , stereochemistry , chromatography , organic chemistry , sulfur , medicine , microbiology and biotechnology , optoelectronics , biology
Hydrogen sulfide (H 2 S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H 2 S in live animals is very challenging. Herein, we report the first radioisotope‐based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H 2 S. Macrocyclic 64 Cu complexes that instantly reacted with gaseous H 2 S to form insoluble 64 CuS in a highly sensitive and selective manner were prepared. The H 2 S concentration in biological samples was measured by a thin‐layer radiochromatography method. When 64 Cu–cyclen was injected into mice, an elevated H 2 S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H 2 S levels in a millimeter‐sized infarcted lesion of the rat heart.