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Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold
Author(s) -
Ke Guoliang,
Liu Minghui,
Jiang Shuoxing,
Qi Xiaodong,
Yang Yuhe Renee,
Wootten Shaun,
Zhang Fei,
Zhu Zhi,
Liu Yan,
Yang Chaoyong James,
Yan Hao
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201603183
Subject(s) - nanobiotechnology , synthetic biology , substrate (aquarium) , nad+ kinase , enzyme , dna origami , dna , nanotechnology , scaffold , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology , computational biology , materials science , computer science , ecology , nanoparticle , database
Artificial multi‐enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD + substrate channeling by specifically shifting NAD + between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.