Premium
Using Single‐Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis
Author(s) -
Li Xianchan,
Dunevall Johan,
Ewing Andrew G.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201602977
Subject(s) - exocytosis , cisplatin , catecholamine , amperometry , chemistry , biophysics , cell , microbiology and biotechnology , medicine , endocrinology , membrane , biochemistry , biology , chemotherapy , electrochemistry , electrode
The pretreatment of cultured pheochromocytoma (PC12) cells with cis ‐diamminedichloroplatinum (cisplatin), an anti‐cancer drug, influences the exocytotic ability of the cells in a dose‐dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single‐cell amperometry reflects that 2 μ m cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 μ m cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.