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Fast Quantitation of Target Analytes in Small Volumes of Complex Samples by Matrix‐Compatible Solid‐Phase Microextraction Devices
Author(s) -
PiriMoghadam Hamed,
Ahmadi Fardin,
GómezRíos German Augusto,
Boyacı Ezel,
ReyesGarcés Nathaly,
Aghakhani Ali,
Bojko Barbara,
Pawliszyn Janusz
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201601476
Subject(s) - solid phase microextraction , analyte , chromatography , matrix (chemical analysis) , sample preparation , complex matrix , mass spectrometry , extraction (chemistry) , chemistry , polypyrrole , materials science , solid phase extraction , analytical chemistry (journal) , gas chromatography–mass spectrometry , polymer , polymerization , organic chemistry
Herein we report the development of solid‐phase microextraction (SPME) devices designed to perform fast extraction/enrichment of target analytes present in small volumes of complex matrices (i.e. V ≤10 μL). Micro‐sampling was performed with the use of etched metal tips coated with a thin layer of biocompatible nano‐structured polypyrrole (PPy), or by using coated blade spray (CBS) devices. These devices can be coupled either to liquid chromatography (LC), or directly to mass spectrometry (MS) via dedicated interfaces. The reported results demonstrated that the whole analytical procedure can be carried out within a few minutes with high sensitivity and quantitation precision, and can be used to sample from various biological matrices such as blood, urine, or Allium cepa L single‐cells.